Cytopathic Effect Inhibition Assay for Canine Interferon Activity.
نویسندگان
چکیده
منابع مشابه
Automated, quantitative cytopathic effect reduction assay for interferon.
A rapid, cytopathic effect reduction assay for human interferon (IFN) is described. Dilutions of IFN were made with an automated diluter in 96-well microtiter plates. Total incubation time was 26 h. IFN titers were calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, which required less than 1 min to read each plate.
متن کاملMicrotiter assay for interferon: microspectrophotometric quantitation of cytopathic effect.
This report describes an accurate, reproducible, and efficient microassay for human interferon, using a dye-uptake method to quantitate cytopathogenicity. The antiviral activity was measured by using a Gilford 300-N microsepctrophotometer, with the automatic programmer, sampler, and data lister. Two hundred interferon samples, each in a final volume of 1.0 ml, may be analyzed and recorded in 45...
متن کاملAssay of interferon activity.
At present there is a lack of standard criteria for the identification and evaluation of activity of antiviral compounds. Interferon was used to explore comparatively several laboratory methods. Interferon was produced in chick embryos and in chorioallantoic membranes suspended in vitro. Evaluation of interferon activity was performed by several methods: (i) percentage of inhibition of plaque-f...
متن کاملCytopathic effect inhibition assay for determining the in-vitro susceptibility of herpes simplex virus to antiviral agents.
We compare a rapid dilution method for the determination of antiviral susceptibility of herpes simplex virus (HSV) with the plaque reduction assay. A total of 84 HSV clinical isolates were studied by both methods to detect in-vitro resistance to acyclovir and foscarnet. The rapid method showed for the detection of HSV isolates resistant to acyclovir and foscarnet, a sensitivity of 96. 8% and 10...
متن کاملHemagglutination - Inhibition Assay For
A sensitive immunoassay procedure for the detection of the thrombopoietic-stimulating factor (TSF) has been developed. This procedure Is similar to the hemagglutination-inhibition (HAl) technique that is available for erythropoietin. For the production of TSF, sheep were made thrombocytopenic by the Injection of antiplatelet serum. TSF from sheep sera was partially purified by column chromatogr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 1996
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.58.23